目录产品 » 稳定细胞系 » Human Recombinant Platelet Activating Factor Receptor Stable Cell Line
CHO-K1/PTAFR Stable Cell Line

Figure 1. PAF-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTAFR cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PAF. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PAF (Mean ± SD, n = 2). The EC50 of PAF on the cells was 0.13 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/PTAFR Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human platelet activating factor receptor (PTAFR) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
M00262
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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human platelet activating factor receptor (PTAFR) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
Applications Calcium mobilization assay, cAMP accumulation assay and IP-One assay.

产品简介
Expressed Gene NCBI reference sequence NM_000952; no expressed tags
Target Protein NP_000943
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)

技术背景
Gene Synonyms PAFR
Background The platelet-activating factor (PAF) receptor (PTAFR) is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and releases of cytokine and leukotriene. In humans, various diseases have been associated with PAF, such as allergic asthma, endotoxic shock, atherosclerosis and psoriasis.

图例
  • CHO-K1/PTAFR Stable Cell Line
    • CHO-K1/PTAFR Stable Cell Line

    Figure 1. PAF-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTAFR cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PAF. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PAF (Mean ± SD, n = 2). The EC50 of PAF on the cells was 0.13 μM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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