| Product Description |
Recombinant CHO-K1 cells stably overexpress human free fatty acid receptor 1 (FFA1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways. |
| Applications |
Calcium mobilization assay, IP-One assay. |
| Expressed Gene |
NCBI reference sequence NM_005303; no expressed tags |
| Target Protein |
NP_005294 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s), 10% FBS |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen) |
| Gene Synonyms |
FFA1R; FFAR1; GPCR40; GPR40 |
| Background |
Free fatty acid G protein coupled receptor family consists of four members and plays significant roles in nutrition regulation. GPR40 (FFA1) and GPR120 are activated by medium and long-chain FFAs, whereas GPR41 and GPR43 (FFA2) can be activated by short-chain FFAs. FFA1 is preferentially expressed in pancreatic beta-cells and mediates the majority of the effects of FFAs on insulin secretion. Researches show that FFA1 is a potential therapeutic target and plays an important role in the chain of events linking obesity and type2 diabetes. |
Figure 1. Sodium oleate-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/FFA1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist sodium oleate. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of sodium oleate (Mean ± SEM, n = 3). The EC50 of sodium oleate on this cell was 20.49 μM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
如您需要获取细胞系产品的说明书，请点击“Manual”，完善信息后点击“提交”。
我们会第一时间联系回复您。注意：中国地区购买的产品，说明书需要以中文站为准。
您也可拨打400-0258686转5810，或发邮件至product@genscript.com.cn联系我们。
更多产品文件类型 >>
