目录产品 » 稳定细胞系 » Human Recombinant H4 Histamine Receptor Stable Cell Line
CHO-K1/H4/Gα15 Stable Cell Line

Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist histamine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of histamine (Mean ± SEM, n = 3). The EC50 of histamine on CHO-K1/H4/Gα15 cell was 38.83 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/H4/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human histamine receptor H4 (H4) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
M00289
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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human histamine receptor H4 (H4) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Applications Calcium flux assay.

产品简介
Expressed Gene NCBI reference sequence NM_021624; no expressed tags
Target Protein NP_067637
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-14, Life Technologies ), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. No. 10131-035, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)

技术背景
Gene Synonyms AXOR35, BG26, GPCR105, GPRv53, H4, H4R, HH4R
Background Histamine receptor family consists of four members, namely, H1, H2, H3, and H4. The histamine H4 receptor is the most recently identified G protein-coupled histamine receptor that binds to several neuroactive drugs, including amitriptyline and clozapine. So far, H4 receptors have been found only on hematopoietic cells. This signifies its important role as a drug target for the treatment of inflammatory diseases.

图例
  • CHO-K1/H4/Gα15 Stable Cell Line
    • CHO-K1/H4/Gα15 Stable Cell Line

    Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist histamine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of histamine (Mean ± SEM, n = 3). The EC50 of histamine on CHO-K1/H4/Gα15 cell was 38.83 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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