目录产品 » 稳定细胞系 » Human Recombinant H4 Histamine Receptor Stable Cell Line
CHO-K1/H4/Gα15 Stable Cell Line

Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist histamine. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Histamine (Mean ± SD, n = 2). The EC50 of histamine on this cell was 50.1 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/H4/Gα15 Stable Cell Line

Histamine receptor family consists of four members, namely, H1, H2, H3, and H4. The histamine H4 receptor is the most recently identified G protein-coupled histamine receptor that binds to several neuroactive drugs, including amitriptyline and clozapine. So far, H4 receptors have been found only on haematopoietic cells. This signifies its important role as a drug target for the treatment of inflammatory diseases.
M00289
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Synonyms

H4 receptor, GPRv53, HH4R, GPCR105, AXOR35, SP9144, H4R, histamine H4 receptor

Applications Functional assay for H4 receptor

Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 400 μg/ml G418 (Cat. #10131-035, Gibco)

  • CHO-K1/H4/Gα15 Stable Cell Line
  • CHO-K1/H4/Gα15 Stable Cell Line

    Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist histamine. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Histamine (Mean ± SD, n = 2). The EC50 of histamine on this cell was 50.1 nM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


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