| Product Description |
Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y
(P2Y2) on the surface and contain high levels of G protein Gαq to couple with
the receptor in downstream signaling pathways. |
| Applications |
Calcium mobilization
assay. |
| Expressed Gene |
Codon optimized from NM_176071;
no expressed tags |
| Target Protein |
NP_788085 |
| Host Cell |
1321N1 |
| Size |
Two vials of frozen
cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages with no significant
changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
45% DMEM (Cat. No. 10569, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No.
D2650, Sigma) |
| Complete Growth Medium |
DMEM,
10% FBS |
| Culture Medium |
DMEM, 10% FBS, 250
μg/ml G418 (Cat.
No. 10131-035, Gibco) |
| Gene Synonyms |
HP2U, P2RU1, P2U,
P2U1, P2UR, P2Y2, P2Y2R |
| Background |
P2Y
receptors are members of a large family of G protein-coupled receptors that is
classified into five functionally characterized human gene products (P2Y1,
P2Y2, P2Y4, P2Y6, and P2Y11).
The
P2Y2 receptor, originally called P2U purinoceptor, recognizes both ATP and UTP
as the most potent agonists. It ranges from excitation of sympathetic neurons,
muscle cell proliferation, endothelial cell adhesion, and spermiogenesis to
acid-base equilibrium in intestinal epithelial cells and mucociliary clearance
in normal and cystic fibrosis airway epithelia. In the airways, the P2Y2 and
the P2Y6 receptors on the apical surface of airway epithelial cells control
several of the calcium and protein kinase C dependent components of mucociliary
clearance. The P2Y2 receptor is also expressed on the basolateral cell surface
along with the P2Y1 receptor. |
Figure 1: ATP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y2 cells. The cells were loaded with Calcium-4 prior to stimulation with a P2Y2 receptor agonist, ATP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ATP (Mean ± SEM, n = 3). The EC50 of ATP on P2Y2 in 1321N1 cells was 32.38 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.
For research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.
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