Figure 1. Neuropeptide S-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPS1b/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist neuropeptide S. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neuropeptide S (Mean ± SEM, n = 3). The EC₅₀ of neuropeptide S on CHO-K1/NPS1b/Gα15 cell was 0.20 μM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with neuropeptide S on CHO-K1/NPS1b/Gα15 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in CHO-K1/NPS1b/Gα15 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Varioskan, Thermo). The EC₅₀ of neuropeptide S on CHO-K1/NPS1b/Gα15 cells was 0.10 μM.

CHO-K1/NPS1b/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human neuropeptide S receptor 1 (NPSR1) isoform B on the surface and contain high levels of G protein Gαs and Gαq to couple with the receptor in downstream signaling pathways.

产品编号	M00344
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant CHO-K1 cells stably overexpress human neuropeptide S receptor 1 (NPSR1) isoform B on the surface and contain high levels of G protein Gαs and Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-14, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1. Neuropeptide S-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPS1b/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist neuropeptide S. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neuropeptide S (Mean ± SEM, n = 3). The EC₅₀ of neuropeptide S on CHO-K1/NPS1b/Gα15 cell was 0.20 μM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with neuropeptide S on CHO-K1/NPS1b/Gα15 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in CHO-K1/NPS1b/Gα15 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Varioskan, Thermo). The EC₅₀ of neuropeptide S on CHO-K1/NPS1b/Gα15 cells was 0.10 μM.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

CHO-K1/NPS1b/Gα15 Stable Cell Line

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