目录产品 » 稳定细胞系 » Human Recombinant G-Protein Coupled Receptor 68 Stable Cell Line
CHO-K1/GPR68/Gα15 Stable Cell Line

Figure 1. H+-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GPR68/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist H+. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of H+ (Mean ± SEM, n = 3). The EC50 of H+ on this cell was 0.24 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/GPR68/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human G protein-coupled receptor 68 (GPR68) on the surface and contain high levels of G protein Gαi and Gαq to couple with the receptor in downstream signaling pathways.
M00439
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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human G protein-coupled receptor 68 (GPR68) on the surface and contain high levels of G protein Gαi and Gαq to couple with the receptor in downstream signaling pathways.
Applications Calcium flux assay.

产品简介
Expressed Gene NCBI reference sequence NM_003485; no expressed tags
Target Protein NP_003476
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)

技术背景
Gene Synonyms AI2A6, GPR12A, OGR1
Background The GPR68 is a proton-sensing receptor involved in pH homeostasis. A study revealed that in osteosarcoma cells and primary human osteoblast precursors which expression of GPR68 exhibit strong pH-dependent inositol phosphate formation.

图例
  • CHO-K1/GPR68/Gα15 Stable Cell Line
    • CHO-K1/GPR68/Gα15 Stable Cell Line

    Figure 1. H+-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GPR68/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist H+. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of H+ (Mean ± SEM, n = 3). The EC50 of H+ on this cell was 0.24 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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