目录产品 » 稳定细胞系 » Human Recombinant ADRA2B Adrenoceptors Stable Cell Line
CHO-K1/ADRA2B/Gqi5 Stable Cell Line

Figure UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2B/Gqi5 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of UK14304 (Mean ± SD, n = 2). The EC50 of UK14304 on this cell was 0.12 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/ADRA2B/Gqi5 Stable Cell Line

Adrenoceptors are 7-transmembrane receptors which mediate the central and peripheral actions of the neurotransmitter, noradrenaline (norepinephrine), and the hormone and neurotransmitter, adrenaline (epinephrine). Based on both pharmacological and molecular evidence, they are divided into three major types - α1, α2, and β. The α2 adrenoceptors include 3 subtypes - α2A, α2B, and α2C - each of which couples to Gi/o protein primarily. The α2B adrenoceptors (ADRA2B) is expressed in the kidney, liver, brain, lung, heart, and skeletal muscle. The development of α2B and α2C knock-out mice has shown that these two subtypes are not involved in the central hypotensive response to α2 agonists.
M00454
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Synonyms

Alpha-2B adrenoceptor, Adrenergic alpha2B- receptor class III, adrenergic receptor, alpha-2B adrenergic receptor, alpha-2B adrenoceptor, alpha-2BAR, alpha2B, [a]2B, Adra-2b

产品概述
Applications Functional assay for ADRA2B receptor

产品简介
Storage Liquid nitrogen immediately upon delivery
Species Human

培养条件
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

图例
  • CHO-K1/ADRA2B/Gqi5 Stable Cell Line
    • CHO-K1/ADRA2B/Gqi5 Stable Cell Line

    Figure UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2B/Gqi5 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of UK14304 (Mean ± SD, n = 2). The EC50 of UK14304 on this cell was 0.12 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
    2. Signal to background Ratio (S/B) = Top/Bottom


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