目录产品 » 稳定细胞系 » Human Recombinant Angiotensin Receptor 1 Stable Cell Line
HEK293/AT1 Stable Cell Line

Figure 1. Angiotensin II-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/AT1 cells. The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to being stimulated with agonist angiotensin II. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of angiotensin II (Mean ± SEM, n = 3). The EC50 of angiotensin II on this cell was 1.23 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

HEK293/AT1 Stable Cell Line

Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with angiotensin II in HEK293/AT1 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in HEK293/AT1 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of angiotension II on HEK293/AT1 cells was 3.53 nM.

HEK293/AT1 Stable Cell Line

Recombinant HEK293 cells stably overexpress human angiotensin II receptor type 1 (AT1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00458
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产品概述
Product Description Recombinant HEK293 cells stably overexpress human angiotensin II receptor type 1 (AT1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Applications Calcium mobilization assay, IP-One assay.

产品简介
Expressed Gene NCBI reference sequence NM_000685; no expressed tags
Target Protein NP_000676
Host Cell HEK293
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 50% DMEM (Cat. No. 10566, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium DMEM, 10% FBS
Culture Medium DMEM, 10% FBS, 3 μg/ml puromycin (Cat. No. A11138-03, Life Technologies)

技术背景
Gene Synonyms AG2SB, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, ATR1, HAT1R, AGTR1
Background Angiotensin receptor 1 (AT1 receptor) is a member of angiotensin receptor which are responsible for the signal transduction of the main effecter hormone. Effects mediated by the AT1 receptor include vasoconstriction, aldosterone synthesis and secretion, increased vasopressin secretion, cardiac hypertrophy, augmentation of peripheral noradrenergic activity, vascular smooth muscle cells proliferation, decreased renal blood flow, renal renin inhibition, renal tubular sodium reuptake, modulation of central sympathetic nervous system activity, cardiac contractility, central osmocontrol and extracellular matrix formation.

图例
  • HEK293/AT1 Stable Cell Line
    • HEK293/AT1 Stable Cell Line

    Figure 1. Angiotensin II-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/AT1 cells. The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to being stimulated with agonist angiotensin II. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of angiotensin II (Mean ± SEM, n = 3). The EC50 of angiotensin II on this cell was 1.23 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

  • HEK293/AT1 Stable Cell Line
    • HEK293/AT1 Stable Cell Line

    Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with angiotensin II in HEK293/AT1 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in HEK293/AT1 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of angiotension II on HEK293/AT1 cells was 3.53 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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