目录产品 » 稳定细胞系 » Human Recombinant Gastric Inhibitory Polypeptide Receptor Stable Cell Line
CHO-K1/GIP/Gα15 Stable Cell Line

Figure 1. GIP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GIP/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist GIP. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GIP (Mean ± SEM, n = 3). The EC50 of GIP on this cell was 0.12 µM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/GIP/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human gastric inhibitory polypeptide receptor (GIP) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
M00486
询价

联系我们
产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human gastric inhibitory polypeptide receptor (GIP) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Applications FACS, calcium mobilization assay, IP-One assay and cAMP accumulation assay.

产品简介
Expressed Gene Codon optimized from NM_000164; no expressed tags
Target Protein NP_000155
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

技术背景
Gene Synonyms GIPR, GIP-R
Background Gastric inhibitory polypeptide receptor GIPR is a Gs-coupled GPCR which is expressed in the pancreas, stomach, small intestine, adipose tissue, adrenal cortex, pituitary, heart, testis, endothelial cells, bone, trachea, spleen, thymus, lung, kidney, thyroid, and several regions in the CNS. Its ligand GIP is secreted after meal ingestion has been shown to stimulate bone formation resulting in lower occurrences of osteoporosis. GIPR may have therapeutic potential in the treatment of type 2 diabetes and obesity.

图例
  • CHO-K1/GIP/Gα15 Stable Cell Line
    • CHO-K1/GIP/Gα15 Stable Cell Line

    Figure 1. GIP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GIP/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist GIP. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of GIP (Mean ± SEM, n = 3). The EC50 of GIP on this cell was 0.12 µM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


喜欢新升级的网站吗？

讨厌

不喜欢

一般

喜欢

非常喜欢

*
Skip