| Product Description |
Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 5 (CXCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways. |
| Applications |
Calcium flux assay, cAMP accumulation assay. |
| Expressed Gene |
NCBI reference sequence NM_001716; no expressed tags |
| Target Protein |
NP_001707 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s), 10% FBS |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies) |
| Gene Synonyms |
BLR1, CD185, MDR15 |
| Background |
Chemokine (C-X-C motif) receptor 5 (CXCR5) belongs to the CXC chemokine receptor family. CXCR5 plays crucial roles in B-cells migration into B-cell follicles of spleen and Peyer patches. CXCR5 reduces maintenance of immature neural cell populations and enhances proliferation of subgranular zone cells in the hippocampal dentate gyrus. GenScript’s human CXCR5-expressing stable subline is guaranteed to function properly in calcium flux assay. |
Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC50 of BCA-1 on CHO-K1/Gα15/CXCR5 cells was 0.11 μM.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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