| Product Description |
Recombinant CHO-K1 cells stably overexpress human programmed cell death 1 (PD-1) on the surface. The surface expression of PD-1 is validated by FACS analysis. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
NCBI reference sequence NM_005018.2; no expressed tags |
| Target Protein |
NP_005009.2 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Gene Synonyms |
PDCD1, AIMTBS, CD279, PD1, SLEB2, hPD-1, hPD-l, hSLE1 |
| Background |
Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein that in humans is encoded by the PDCD1 gene. PD-1, functioning as an immune checkpoint, plays an important role in down regulating the immune system by preventing the activation of T-cells, which in turn reduces autoimmunity and promotes self-tolerance. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells (suppressor T cells). |
Figure 1. FACS analysis of cell surface expression of human PD-1 on CHO-K1/PD-1 cells. The CHO-K1/PD-1 cells (blue) and the negative control CHO-K1 cells (red) were probed using APC-conjugated anti-human CD279 (Cat. No. 329908, Biolegend).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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