目录产品 » 稳定细胞系 » CHO-K1/VISTA
CHO-K1/VISTA Stable Cell Line

Figure 1: FACS analysis of cell surface expression of VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green and blue) were probed using PE-human VISTA (Cat. No. FAB71261P, R&D).

CHO-K1/VISTA Stable Cell Line

Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.
M00533
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.
Culture Properties Adherent
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)
Complete Growth Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Freeze Medium-DATA 95% Complete growth medium, 5% DMSO (Cat. No.D2650, Sigma)

图例
  • CHO-K1/VISTA Stable Cell Line
    • CHO-K1/VISTA Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green and blue) were probed using PE-human VISTA (Cat. No. FAB71261P, R&D).


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