| Product Description |
Recombinant CHO-K1 cells stably overexpress human CD276 (B7-H3) on the surface. The surface expression of B7-H3 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against B7-H3 or to measure binding affinity between B7-H3 and anti-B7-H3 antibodies. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
NCBI reference sequence NM_001024736.1; no expressed tags |
| Target Protein |
NP_001019907.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 6 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Gene Synonyms |
CD276, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2 |
| Background |
B7-H3, a new member of the B7 family of immune-regulatory molecules, was identified in 2001 by database searches of a human dendritic cell-derived cDNA library. It is a type I transmembrane protein, which expresses in certain normal cells and tissues, such as dendritic cells, as well as the liver, lung, breast, placenta, and prostate. Aberrant expression of B7-H3 has been reported in a wide range of solid cancers, including brain, lung, pancreatic, colorectal, liver, and breast cancers, as well as in hematologic malignancies, such as acute leukemia and multiple myeloma, and it is associated with more advanced disease and poor prognosis. |
Figure 1. FACS analysis of cell surface expression of human B7-H3 on CHO-K1/B7-H3 cells. The CHO-K1/B7-H3 cells (blue) and the negative control CHO-K1 cells (red) were probed using anti-human B7-H3 mouse IgG (Cat. No. MAB1027, R&D systems) and FITC-conjugated goat anti-mouse IgG (Cat. No. sc-2010, Santa Cruz).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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