| Product Description |
Recombinant CHO-K1 cells stably overexpress human programmed cell death 1 ligand 1 (PD-L1) on the surface. The surface expression of PD-L1 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against PD-L1 or to measure binding affinity between PD-L1 and anti-PD-L1 antibodies. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
NCBI reference sequence NM_014143; no expressed tags |
| Target Protein |
NP_054862.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Gene Synonyms |
CD274; B7-H1; B7H1; PDCD1L1; PDCD1LG1; PDL1 |
| Background |
Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. The formation of PD-1 receptor/PD-L1 or B7.1 receptor/PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2 |
Figure 1: FACS analysis of cell surface expression of PD-L1 on CHO-K1 cells. The CHO-K1/PD-L1 cells (red) and the negative control CHO-K1 cells (green) were probed using Anti-Human PD-L1 (GenScript) and Alexa Fluor™ 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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