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HEK293/Gα15 Stable Cell Line

Figure 1. Intracellular calcium mobilization of 10 μM VIP on transient-transfected HEK293/Gα15 cells with pcDNA3.1-VPAC2 plasmid.

HEK293/Gα15 Stable Cell Line

HEK293/Gα15 is a HEK293 cell line stably expressing the Gα15 alpha subunit protein which a Gq protein.  It is used as a host cell for transfection expression of Gs and Gi/o -coupled receptors, the constitutively expressed Gα15 protein in the cells allows many transfected receptors which normally stimulate/inhibit the cAMP pathway, to couple to Gq signal transduction and mobilize intracellular calcium.  The cell line carries the puromycin resistance gene and is resistant to puromycin
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Description

HEK293/Gα15 is a HEK293 cell line stably expressing the Gα15 alpha subunit protein which a Gq protein.  It is used as a host cell for transfection expression of Gs and Gi/o -coupled receptors, the constitutively expressed Gα15 protein in the cells allows many transfected receptors which normally stimulate/inhibit the cAMP pathway, to couple to Gq signal transduction and mobilize intracellular calcium.  The cell line carries the puromycin resistance gene and is resistant to puromycin

Synonyms

GNA15, GNA16

Applications 16 passages

Storage Liquid nitrogen immediately upon delivery

Freeze Medium 90% FBS, 10% DMSO
Culture Medium DMEM, 10% FBS, 0.5 μg/ml puromycin

  • HEK293/Gα15 Stable Cell Line
  • HEK293/Gα15 Stable Cell Line

    Figure 1. Intracellular calcium mobilization of 10 μM VIP on transient-transfected HEK293/Gα15 cells with pcDNA3.1-VPAC2 plasmid.


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