目录产品 » 稳定细胞系 » CHO-K1/Gα15/AMY1 Stable Cell Line
CHO-K1/Gα15/AMY1 Stable Cell Line

Figure 1. Calcitonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/AMY1 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with AMY1 agonist, calcitonin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of calcitonin (mean ± SEM, n = 3). The EC50 of calcitonin on CHO-K1/Gα15/AMY1 cells was 0.11 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

CHO-K1/Gα15/AMY1 Stable Cell Line

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with amylin in CHO-K1/Gα15/AMY1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/Gα15/AMY1 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of amylin on CHO-K1/Gα15/AMY1 cells was 4.94 nM.

CHO-K1/Gα15/AMY1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human receptor activity modifying protein 1 (RAMP1) and calcitonin receptor (CTR) on the surface. Amylin receptors (AMYR) are a class of special protein heterodimers, composed of a CTR combined with one of three homologous receptor activity-modifying proteins (RAMP1, RAMP2, RAMP3), forming three subtypes (AMY1, AMY2, AMY3). This cell contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
M00557
询价

联系我们
Product Description Recombinant CHO-K1 cells stably overexpress human receptor activity modifying protein 1 (RAMP1) and calcitonin receptor (CTR) on the surface. Amylin receptors (AMYR) are a class of special protein heterodimers, composed of a CTR combined with one of three homologous receptor activity-modifying proteins (RAMP1, RAMP2, RAMP3), forming three subtypes (AMY1, AMY2, AMY3). This cell contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

  • CHO-K1/Gα15/AMY1 Stable Cell Line
  • CHO-K1/Gα15/AMY1 Stable Cell Line

    Figure 1. Calcitonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/AMY1 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with AMY1 agonist, calcitonin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of calcitonin (mean ± SEM, n = 3). The EC50 of calcitonin on CHO-K1/Gα15/AMY1 cells was 0.11 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

  • CHO-K1/Gα15/AMY1 Stable Cell Line
  • CHO-K1/Gα15/AMY1 Stable Cell Line

    Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with amylin in CHO-K1/Gα15/AMY1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/Gα15/AMY1 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of amylin on CHO-K1/Gα15/AMY1 cells was 4.94 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


喜欢新升级的网站吗?

讨厌

不喜欢

一般

喜欢

非常喜欢

*