| Product Description |
Recombinant CHO-K1 cells stably overexpress human
OX-40L on the surface. The surface expression of OX-40L is validated by FACS
analysis. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
Codon optimized from
NM_003326.4; no expressed tags |
| Target Protein |
NP_003317.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen
cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages without significant
changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95%
complete growth medium, 5% (V/V)
DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022,
Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
| Gene Synonyms |
CD134L,
CD252, GP34, OX-40L, OX40L, TNLG2B, TXGP1 |
| Background |
OX-40L is
the ligand for CD134 and is expressed on such cells as DC2s (a subtype of
dendritic cells) enabling amplification of Th2 cell differentiation. OX-40L has
also been designated CD252 (cluster of differentiation 252). Various single
nucleotide polymorphisms (SNPs) of the OX-40L gene have been identified. For
some of them association with systemic lupus erythematosus has been reported. |
Figure 1. WB analysis of OX-40L expression in CHO-K1/OX-40L and CHO-K1 cells. The CHO-K1/OX-40L cells and the negative control CHO-K1 cells were lysed and detected with PE labeled anti-human CD252 (OX40L) Antibody (Cat. No. 326308, Biolegend).
For research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.
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