| Product Description |
Recombinant CHO-K1 cells stably overexpress
mouse programmed cell death 1 ligand 1 (PD-L1) on the surface. The surface
expression of PD-L1 is validated by FACS analysis. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
Codon optimized from NM_021893.3;
no expressed tags |
| Target Protein |
NP_068693.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen
cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318,
Lonza). |
| Stability |
Stable through more than 16 passages
without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95%
complete growth medium, 5% (V/V)
DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco),
10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin
(Cat. No. A11138-03, Gibco) |
| Gene Synonyms |
CD274;
B7-H1; B7H1; PDCD1L1; PDCD1LG1; PDL1 |
| Background |
Programmed death-ligand 1 (PD-L1) also known as cluster of
differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans
is encoded by the CD274 gene. The formation of PD-1 receptor / PD-L1 or B7.1
receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the
proliferation of these CD8+ T cells at the lymph nodes and supplementary to that
PD-1 is also able to control the accumulation of foreign antigen specific T
cells in the lymph nodes through apoptosis which is further mediated by a lower
regulation of the gene Bcl-2 |
FACS analysis of mouse PD-L1 expression in CHO-K1/mouse PD-L1 cells.
For research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.
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