目录产品 » 稳定细胞系 » Mouse Recombinant CTLA4 Stable Cell Line
CHO-K1/mouse CTLA4 Stable Cell Line

Figure 1: FACS analysis of cell surface expression of mouse CTLA4 on CHO-K1/mouse CTLA4 cells. The CHO-K1/mouse CTLA4 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE anti-mouse CD152 (Cat. No. 106305, Biolegend).

CHO-K1/mouse CTLA4 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress Mus musculus cytotoxic T-lymphocyte-associated protein 4 (CTLA4) on the surface. The surface expression of mouse CTLA4 is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse CTLA4 and for evaluating target binding affinity.
M00570
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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress Mus musculus cytotoxic T-lymphocyte-associated protein 4 (CTLA4) on the surface. The surface expression of mouse CTLA4 is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse CTLA4 and for evaluating target binding affinity.  
Applications Cell-based binding assay or use as immunogen

产品简介
Expressed Gene Codon optimized from NM_009843.4; no expressed tags
Target Protein NP_033973
Host Cell CHO-K1
Quantity 2 vials of frozen cells (1x106 per vial in 1 mL) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza).
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 95% Culture Medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

技术背景
Gene Synonyms Cd152; Ly-56; Ctla-4
Background CTLA4 or CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152 (cluster of differentiation 152), is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses. CTLA4 is constitutively expressed in Tregs but only upregulated in conventional T cells after activation. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.

图例
  • CHO-K1/mouse CTLA4 Stable Cell Line
    • CHO-K1/mouse CTLA4 Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of mouse CTLA4 on CHO-K1/mouse CTLA4 cells. The CHO-K1/mouse CTLA4 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE anti-mouse CD152 (Cat. No. 106305, Biolegend).


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