| Product Description |
Recombinant CHO-K1 cells stably overexpress human
CD16A and Fc epsilon receptor Ig (FcER1G) on the surface. The surface
expression of CD16A is validated by FACS analysis. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
Codon optimized from NM_000569.6
and NM_004106.2; no expressed tags |
| Target Protein |
NP_000560.5 and
NP_004097.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen
cells (>1×106 per
vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318,
Lonza). |
| Stability |
Stable through more than 15 passages
without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth
medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco),
10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin
(Cat. No. A11138-03, Gibco), 100 μg/ml
Hygromycin B (Cat.
No.10687010, Invitrogen) |
| Gene Synonyms |
CD16A, FCGR3A, FCG3, FCGR3, (for CD16A);
FcER1G, FCGR, (for FcER1G) |
| Background |
CD16 is a
cluster of differentiation molecule found on the surface of natural killer
cells, monocytes and macrophages. It can be used to isolate populations of
these cells by antibodies directed towards CD16, using fluorescent-activated
cell sorting or magnetic-activated cell sorting. CD16 has been identified as Fc
receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). The two isoforms of the CD16a
stimulatory receptor found on natural killer (NK) cells and macrophages differ
by one amino acid at codon 158 (V/F) and are important for antibody-dependent
cell-mediated cytotoxicity (ADCC). The high affinity 158 V/V homodimer of CD16A
is present on approximately 20% of the population and the lower affinity F/F
homodimer and F/V heterodimer are present in the remaining 80%. |
Figure 1: FACS analysis of cell surface expression of CD16A 158F on CHO-K1/CD16A 158F cells.
The CHO-K1/CD16A 158F cells (blue) and the negative control CHO-K1 cells (red) were stained with PerCP/Cyanine5.5 anti-human CD16 Antibody (Cat. No. 302028, Biolegend).
For research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.
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