| Product Description |
Recombinant CHO-K1 cells stably overexpress human
CD16A and Fc epsilon receptor Ig (FcER1G) on the surface. The surface
expression of CD16A is validated by FACS analysis. |
| Culture Properties |
Adherent |
| Stability |
Stable through more than 15 passages
without significant changes in assay performance or expression profile. |
| Size |
Two vials of frozen
cells (>1×106 per
vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin
(Cat. No. A11138-03, Gibco), 100 μg/ml
Hygromycin B (Cat.
No.10687010, Invitrogen) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco),
10% FBS (Cat. No. 10099-141, Gibco) |
| Freeze Medium-DATA |
95% complete growth
medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
Figure 1: FACS analysis of cell surface expression of CD16A 158F on CHO-K1/CD16A 158F cells.
The CHO-K1/CD16A 158F cells (blue) and the negative control CHO-K1 cells (red) were stained with PerCP/Cyanine5.5 anti-human CD16 Antibody (Cat. No. 302028, Biolegend).
For research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.
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