Figure 1: FACS analysis of cell surface expression of CD32B 232Ile on CHO-K1 cells.
CHO-K1/CD32B 232Ile cells (blue) and negative control CHO-K1 cells (red) were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 303208, Biolegend).

Figure 2: FACS analysis of cell surface expression of CD32B 232Ile on CHO-K1 cells from different passages.
CHO-K1/CD32B 232Ile cells and negative control CHO-K1 cells were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Biosciences). P17, P23, P28, and P33 represent cell passage numbers.

CHO-K1/CD32B Ile232 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-b (FcγRIIb/CD32B 232Ile) on the surface. The surface expression of CD32B 232Ile is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32B 232Ile. GenScript also offers CD32B 232Thr stable cell line (Cat. No. M00600) for FcγRIIb polymorphism study.

产品编号	M00587
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-b (FcγRIIb/CD32B 232Ile) on the surface. The surface expression of CD32B 232Ile is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32B 232Ile. GenScript also offers CD32B 232Thr stable cell line (Cat. No. M00600) for FcγRIIb polymorphism study.
Culture Properties	Adherent
Stability	Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

图例

Figure 1: FACS analysis of cell surface expression of CD32B 232Ile on CHO-K1 cells.
CHO-K1/CD32B 232Ile cells (blue) and negative control CHO-K1 cells (red) were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 303208, Biolegend).

Figure 2: FACS analysis of cell surface expression of CD32B 232Ile on CHO-K1 cells from different passages.
CHO-K1/CD32B 232Ile cells and negative control CHO-K1 cells were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Biosciences). P17, P23, P28, and P33 represent cell passage numbers.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

CHO-K1/CD32B Ile232 Stable Cell Line

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