CHO-K1/CD32B Ile232 Stable Cell Line

Product Description

Recombinant CHO-K1 cells stably overexpress low affinity immunoglobulin gamma Fc region receptor II-b (FcγRIIb / CD32B 232Ile). The surface expression of CD32B 232Ile is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of antibody based biologics binding with CD32B 232Ile. GenScript offers both CD32B 232Ile expressing stable cell line and CD32B 232Thr expressing stable cell line (Cat. No. M00600) for FcγRIIb polymorphism study.

Applications

Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon optimized from NM_004001.4; no expressed tags
Target Protein	NP_003992.3
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1X10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	F-12K (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	F-12K, 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

技术背景

Gene Synonyms

FCGR2B; FCG2; IGFR2; CD32

Background

Human CD32 group consists of FcγRIIa, b and c proteins that share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32B, also known as FcγRIIb, is a low affinity receptor that recognizes immunoglobulin gamma complexes. It is encoded by the FCGR2B gene and expressed on B cells and most myeloid cells. CD32B has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Co-ligation of CD32B with B cells antigen receptor (BCR) results in selective dephosphorylation of CD19 tyrosine, tyrosine phosphorylation of the ITIM in the CD32B cytoplasmic domain, and recruitment of SH2-containing inositol 5-phosphatase (SHIP). This causes a block in calcium influx, inhibition of B cell activation, and leads to apoptosis. CD32B is a potential target for monoclonal antibody therapy for malignancies. Targeting CD32B may represent a novel approach to immunotherapy of B-cell malignancies.

图例

Figure 1: FACS analysis of cell surface expression of CD32B 232Ile on CHO-K1 cells.
CHO-K1/CD32B 232Ile cells (blue) and negative control CHO-K1 cells (red) were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 303208, Biolegend).

Figure 2: FACS analysis of cell surface expression of CD32B 232Ile on CHO-K1 cells from different passages.
CHO-K1/CD32B 232Ile cells and negative control CHO-K1 cells were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Biosciences). P17, P23, P28, and P33 represent cell passage numbers.

产品编号	M00587
规格	2 vials 大单询价
数量
价格	询价


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