| Product Description |
Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-b (FcγRIIb/CD32B 232Ile) on the surface. The surface expression of CD32B 232Ile is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32B 232Ile. GenScript also offers CD32B 232Thr stable cell line (Cat. No. M00600) for FcγRIIb polymorphism study. |
| Applications |
Binding assay or use as immunogen for antibody development |
| Expressed Gene |
NCBI reference sequence NM_004001.4 |
| Target Protein |
NP_003992.3 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Gene Synonyms |
CD32, CD32B, FCG2, FCGR2, FCGR2C, FcGRIIB, FcRII-c, FcgammaRIIb, IGFR2 |
| Background |
Human CD32 group consists of FcγRIIa, b and c proteins that share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32B is a low affinity receptor that recognizes immunoglobulin gamma complexes. It is expressed on B cells and most myeloid cells. CD32B has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Co-ligation of CD32B with B cells antigen receptor (BCR) results in selective dephosphorylation of CD19 tyrosine, tyrosine phosphorylation of the ITIM, and recruitment of SH2-containing inositol 5-phosphatase (SHIP). This causes a block in calcium influx, inhibition of B cell activation, and leads to apoptosis. CD32B is a potential target for monoclonal antibody therapy for malignancies. Targeting CD32B may represent a novel approach to immunotherapy of B-cell malignancies. |
Figure 1: FACS analysis of cell surface expression of CD32B 232Ile on CHO-K1 cells.
CHO-K1/CD32B 232Ile cells (blue) and negative control CHO-K1 cells (red) were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 303208, Biolegend).
Figure 2: FACS analysis of cell surface expression of CD32B 232Ile on CHO-K1 cells from different passages.
CHO-K1/CD32B 232Ile cells and negative control CHO-K1 cells were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Biosciences). P17, P23, P28, and P33 represent cell passage numbers.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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