| Product Description |
Recombinant CHO-K1 cells stably overexpress human C-type lectin domain family 4 member C (BDCA2) and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of BDCA2 is validated by FACS analysis. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
NCBI reference sequence NM_130441.1 and NM_004106.1; no expressed tags |
| Target Protein |
NP_569708.1 and NP_004097.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Gene Synonyms |
CLEC4C; DLEC; HECL; CD303; BDCA-2; CLECSF7; CLECSF11; PRO34150 (for BDCA2); FCRG (for FcER1G) |
| Background |
BDCA-2 is a novel type II C-type lectin, which shows 50.7% sequence identity at the amino acid level to its putative murine ortholog, the murine dendritic cell–associated C-type lectin 2. In addition to its Ag-capturing function, BDCA-2 can mediate potent inhibition of induction of IFN-α/β expression in PDCs. Production of IFN-α/β in response to several different types of viruses, bacteria, CpG-DNA, dsRNA, and SLE serum is by far the most prominent feature of PDCs. |
Figure 1. FACS analysis of human BDCA2 expression in CHO-K1/BDCA2/FcER1G cells. The CHO-K1/BDCA2/FcER1G cells (blue) and the negative control CHO-K1 cells (red) were probed using PE conjugated Anti-BDCA2 (Cat. No. 130-090-511, Miltenyi).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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