| Product Description |
Recombinant CHO-K1 cells stably overexpress human low affinity immunoglobulin gamma Fc region receptor III-A (FcγRIIIa/CD16A 158V) and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of CD16A 158V is validated by FACS analysis. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
NCBI reference sequence NM_000569.6 (for CD16A) and NM_004106.1 (for FcER1G); no expressed tags |
| Target Protein |
NP_000560.5 and NP_004097.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318,Lonza). |
| Stability |
Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No.10687010, Life Technologies) |
| Gene Synonyms |
CD16A FCGR3A, FCG3, FCGR3 (for CD16A); FcER1G; FCRG (for FcER1G) |
| Background |
Recombinant CHO-K1 cells stably express low affinity immunoglobulin gamma Fc region receptor III-A (FcγRIIIa/CD16A 158V) complex on the cell surface, including 1 alpha chain and 2 gamma chains. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16A 158V. GenScript also offers a stable cell line expressing the CD16A of 158F allotype (Cat. No. M00586) for FcγRIIIa polymorphism study.
CD16 is a cluster of differentiation molecule found on the surface of natural killer cells, monocytes and macrophages. It can be used to isolate populations of these cells by antibodies directed towards CD16, using fluorescent-activated cell sorting or magnetic-activated cell sorting. CD16 has been identified as CD16A and CD16B. The two isoforms of the CD16A stimulatory receptor found on natural killer (NK) cells and macrophages differ by one amino acid at codon 158 (V/F) and are important for antibody-dependent cell-mediated cytotoxicity (ADCC). The high affinity 158 V/V homodimer of CD16A is present on approximately 20% of the population and the lower affinity F/F homodimer and F/V heterodimer are present in the remaining 80%. |
Figure 1: FACS analysis of cell surface expression of human CD16A 158V on CHO-K cells.
CHO-K1/CD16A 158V cells (blue) and negative control CHO-K1 cells (red) were stained with PE-Cy™5 Mouse Anti-Human CD16 antibody (Cat. No. 555408, BD Bioscience).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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