| Product Description |
Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-b (FcγRIIb/CD32B 232Thr) on the surface. The surface expression of CD32B 232Thr is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32B 232Thr. GenScript also offers CD32B 232Ile stable cell line (Cat. No. M00587) for FcγRIIb polymorphism study. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
NCBI reference sequence KR710627.1 |
| Target Protein |
AKI71009.1 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Gene Synonyms |
CD32, CD32B, FCG2, FCGR2, FCGR2C, FcGRIIB, FcRII-c, FcgammaRIIb, IGFR2 |
| Background |
FCGR2B (CD32B) is a low affinity receptor for Immunoglobulin G (IgG). Mutation in the gene in humans leads to a lupus phenotype. The cytoplasmic part of this receptor contains an immunoreceptor tyrosine-based inhibitory motif, in contrast to the activating isoform, FCGR2A (CD32A). |
Figure 1: FACS analysis of cell surface expression of CD32B 232 Thr on CHO-K1/CD32B 232 Thr cells.
The CHO-K1/CD32B 232 Thr cells (blue) and the negative control CHO-K1 cells (red) were stained with APC anti-human CD32 antibody (Cat. No. 303208, BioLegend).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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