Product Description |
Recombinant CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb/CD16B NA1). The surface expression of CD16B NA1 is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16B during biologics research and development. |
Culture Properties |
Adherent |
Stability |
Stable through more than 15 passages without significant changes in assay performance or expression profile. |
Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
Freeze Medium-DATA |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |

Figure 1. FACS analysis of cell surface expression of CD16B NA1 on CHO-K1/CD16B NA1 cells. The CHO-K1/CD16B NA1 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated mouse anti-human CD16 (Cat. No. 302008, Biolegend).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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