| Product Description |
Recombinant CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb/CD16B NA1). The surface expression of CD16B NA1 is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16B during biologics research and development. |
| Applications |
Binding assay or use as immunogen for antibody development |
| Expressed Gene |
NCBI reference sequence CCDS41433.1; no expressed tags |
| Target Protein |
NP_000561.3 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Gene Synonyms |
FCGR3B, CD16, CD16-I, CD16b, FCG3, FCGR3, FCR-10, FCRIII, FCRIIIb |
| Background |
CD16 has been identified as FcγRIIIa (CD16A) and FcγRIIIb (CD16B) which are similar to each other. CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells. CD16B exists as a monomeric glycosylphosphatidylinositol (GPI)-anchored form in neutrophils. CD16B (FcγRIIIb) exists in two allelic forms, NA1 and NA2. The polymorphism of CD16B exhibits different binding affinities for IgG1 and IgG3 subclasses. The different allelic versions are also demonstrated to be associated with susceptibility to diseases like idiopathic pulmonary fibrosis and systemic lupus. |
Figure 1. FACS analysis of cell surface expression of CD16B NA1 on CHO-K1/CD16B NA1 cells. The CHO-K1/CD16B NA1 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated mouse anti-human CD16 (Cat. No. 302008, Biolegend).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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