| Product Description |
Recombinant CHO-K1 cells stably overexpress human FcRn and B2M subunit on the surface. The B2M subunits enable stable expression of FcRn in cells. Association of FcRn alpha-chain with beta (2) m is also important for IgG binding. The surface expression of FcRn is validated by FACS analysis. |
| Applications |
Binding assay or use as immunogen |
| Expressed Gene |
NCBI reference sequence NM_004107 (for FcRn) and AF072097 (for B2M); no expressed tags |
| Target Protein |
NP_004098.1 (for FcRn) and AAD48083.1 (for B2M) |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. A5669701, Life Technologies) |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 200 μg/ml Hygromycin B (Cat. No.10687010, Life Technologies) |
| Gene Synonyms |
FCGRT, alpha-chain, FcgammaRn (for FcRn), and Beta-2-Microglobulin (for B2M) |
| Background |
This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. |
Figure 1. FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells.
The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were probed using FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz Biotechnology) and PE conjugated Goat anti-mouse IgG (minimal x-reactivity) Antibody (Cat. No. 405307, Biolegend).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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