目录产品 » Human Recombinant 4-1BB Stable Reporter Cell Line
HT1080/4-1BB Stable Reporter Cell Line

Flow cytometry analysis of 4-1BB protein expression in HT1080/4-1BB cells.
Expression of 4-1BB was detected with anti-4-1BB antibody.
Red: HT1080 cells,
Blue: HT1080/4-1BB cells.

HT1080/4-1BB Stable Reporter Cell Line

Functional analysis of 4-1BBL on HT1080/4-1BB cells by measuring IL-8 release.

HT1080/4-1BB Stable Reporter Cell Line

4-1BB is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), CD137 and induced by lymphocyte activation (ILA). It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule. 4-1BB can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of 4-1BB is its costimulatory activity for activated T cells. Crosslinking of 4-1BB enhances T cell proliferation, IL-2 secretion, survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.
M00609
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Description

4-1BB is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), CD137 and induced by lymphocyte activation (ILA). It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule.
4-1BB can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, 4-1BB expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
The best characterized activity of 4-1BB is its costimulatory activity for activated T cells. Crosslinking of 4-1BB enhances T cell proliferation, IL-2 secretion, survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.
HT1080 is derived from human fibrosacoma biopsy. It is highly active in NF-kB signaling pathway which is necessary for immune responses. HT1080 cells overexpressed with 4-1BB are significantly increased in immune response indicated by elevated production of IL-8.

Synonyms

CD137; ILA; TNFRSF9

Applications Reporter cell line for 4-1BB functional assay. Assay readout IL-8.

Storage Liquid nitrogen immediately upon receiving
QC Mycoplasma negative, stability tested for 20 passages
Species Human
Stability tested for 20 passages
Volume 1ml in each vial, 2 vials

Freeze Medium 95% complete growth medium, 5% DMSO
Culture Medium MEM, 10% FBS, 200 μg/ml Hygromycin B, 2 μg/ml Puromycin

  • HT1080/4-1BB Stable Reporter Cell Line
  • HT1080/4-1BB Stable Reporter Cell Line

    Flow cytometry analysis of 4-1BB protein expression in HT1080/4-1BB cells.
    Expression of 4-1BB was detected with anti-4-1BB antibody.
    Red: HT1080 cells,
    Blue: HT1080/4-1BB cells.

  • HT1080/4-1BB Stable Reporter Cell Line
  • HT1080/4-1BB Stable Reporter Cell Line

    Functional analysis of 4-1BBL on HT1080/4-1BB cells by measuring IL-8 release.


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