Figure 1: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells.
CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

Figure 2: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells from different passages.
The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 represent cell passage numbers.

CHO-K1/ACE2 Stable Cell Line

Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure binding affinity and stability of antibody based biologics binding with ACE2.

产品编号	M00771
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure binding affinity and stability of antibody based biologics binding with ACE2.
Culture Properties	Adherent
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	F-12K, 10% FBS, 250 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)
Complete Growth Medium	F-12K (Cat. # 21127-022, Gibco), 10% FBS (Cat. #10099-141, Gibco)
Freeze Medium-DATA	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells.
CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

Figure 2: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells from different passages.
The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 represent cell passage numbers.

CHO-K1/ACE2 Stable Cell Line

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