目录产品 » Human Recombinant SARS-CoV-2 Spike Stable Cell Line
CHO-K1/Spike Stable Cell Line

Figure 1: FACS analysis of cell surface expression of SARS-CoV-2 spike protein on CHO-K1 cells. CHO-K1/Spike cells (red) and negative control CHO-K1 cells (grey) were probed using ACE-2 Fc Chimera, Human (Cat. No. Z03516, GenScript) and Alexa Fluor 647 conjugated Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Cat. No. A-21445, Thermo Fisher).

CHO-K1/Spike Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human SARS-CoV-2 spike protein on their surface. The surface expression of SARS-CoV-2 spike protein is validated by FACS analysis. This stable cell line product is designed for screening antibodies against the SARS-CoV-2 spike protein, as well as measuring binding affinity and stability of antibody-based biologics that bind with spike protein. GenScript also offers spike protein expressing HEK293T stable cell line (Cat. No. M00804) for SARS-CoV-2 study.
M00803
询价

联系我们
产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human SARS-CoV-2 spike protein on their surface. The surface expression of SARS-CoV-2 spike protein is validated by FACS analysis. This stable cell line product is designed for screening antibodies against the SARS-CoV-2 spike protein, as well as measuring binding affinity and stability of antibody-based biologics that bind with spike protein. GenScript also offers spike protein expressing HEK293T stable cell line (Cat. No. M00804) for SARS-CoV-2 study.
Culture Properties Adherent
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile. 
Size 2 vials of frozen cells (>1X106 per vial in 1 mL) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham's F-12K (Kaighn's) Medium, 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/mL Puromycin (Cat. No. A11138-03, Gibco) 
Complete Growth Medium Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Freeze Medium-DATA 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/Spike Stable Cell Line
    • CHO-K1/Spike Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of SARS-CoV-2 spike protein on CHO-K1 cells. CHO-K1/Spike cells (red) and negative control CHO-K1 cells (grey) were probed using ACE-2 Fc Chimera, Human (Cat. No. Z03516, GenScript) and Alexa Fluor 647 conjugated Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Cat. No. A-21445, Thermo Fisher).


喜欢新升级的网站吗？

讨厌

不喜欢

一般

喜欢

非常喜欢

*
Skip