| Product Description |
Recombinant HEK293T cells stably overexpress coronavirus SARS-CoV-2 spike protein on the surface. The surface expression of SARS-CoV-2 spike protein is validated by FACS analysis. This stable cell line product is designed for screening antibodies against SARS-CoV-2 spike protein, as well as for measuring binding affinity and stability of antibody based biologics that bind with spike protein. GenScript also offers spike protein expressing CHO-K1 stable cell line (Cat. No. M00803) for SARS-CoV-2 study. |
| Applications |
Cell-based binding assay; use as immunogen for antibody development. |
| Expressed Gene |
NCBI reference sequence NC_045512.2; no expressed tags |
| Target Protein |
YP_009724390.1 |
| Host Cell |
HEK293T |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
50% DMEM (Cat. No. 10569, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 5% DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
DMEM, 10% FBS |
| Culture Medium |
DMEM, 10% FBS, 1 μg/ml puromycin (Cat. No. A11138-03, Life Technologies) |
| Gene Synonyms |
S; Spike glycoprotein |
| Background |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell, the infecting RNA is used to encode structural proteins that make virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control, and accessory proteins whose function has not been determined. The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M), and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronaviruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. |
Figure 1: FACS analysis of cell surface expression of SARS-CoV-2 spike protein on HEK293T cells. HEK293T/Spike cells (blue) and negative control HEK293T cells (grey) were probed using ACE-2 Fc Chimera, Human (Cat. No. Z03516, GenScript) and Alexa Fluor 647 conjugated Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Cat. No. A-21445, Thermo Fisher).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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