FACS analysis of CLDN18.2 expression in CHO-K1/ CLDN18.2 clone by antiCLDN18.2, compared with blank control (green line, untreated cell) and negative control (blue line, only treated with goat anti-human antibody). The cells analyzed are live cells (E1 gate).

Cell based ELISA of CLDN18.2 expression in CHO-K1/ CLDN18.2 clone. CHO-K1/CLDN18.2 cells are plated at 8E4 cells /well in 100μl DMEM plus with 10% FBS in 96-well plate overnight. Then fix with paraformaldehyde for 15 minutes. After washed, the cells are incubated with concentration gradient of IMAB-362 (anti-CLDN18.2 antibody) for 1 hour, and incubated with goat anti-human IgG Antibody (H&L) [HRP] antibody. After incubated with TMB, OD450 is detected by microplate reader

Immune assay of CLDN18.2 expression in CHO-K1/ CLDN18.2 clone. CHO-K1/ CLDN18.2 cells are plated at 5E5 cells /well in 100μl PBS in 96-well plate, incubated with concentration gradient of IMAB-362(anti-CLDN18.2 antibody) on ice for 1 hour. Then discard the supernatant and incubated with goat anti-human IgG antibody at 10 μg/ml on ice for 1 hour. Then analyzed on flow cytometer and analysis the mean fluorescence intensity of APC (Mean APC-H) in live cell gate (E1 in Figure 1).

Stability testing of CLDN18.2 expression in CHO-K1/ CLDN18.2 clone.4 for 22 passages by anti-CLDN18.2, compared with blank control (blue line, untreated cell) and negative control (purple line, treated with goat anti-human antibody)

CHO-K1/CLDN18.2 Stable Cell Line

Claudins are a family of proteins, which play a role in maintaining tight cell junctions. Differentclaudin subtypes are expressed on different tissues.Claudin 18 isoform 2 (CLDN18.2) expressionin normal tissues is restricted to cells of the gastric mucosa and is absent from other healthytissues However, CLDN 18.2 is expressed in 70% of primary gastric adenocarcinomas and itsmetastases (101). It has also been shown to be expressed in a number of other cancers,including pancreatic (50%), esophagus (30%) and NSCLC (25%)The restricted expressionmakes it a potential drug target for the treatment of gastric and pancreatic adenocarcinoma, asevidenced by efforts to target CLDN18.2 via naked antibody and CAR-T modalities.

产品编号	M00916
规格	2 vials 大单询价
数量
价格	询价


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Description

Claudins are a family of proteins, which play a role in maintaining tight cell junctions. Differentclaudin subtypes are expressed on different tissues.Claudin 18 isoform 2 (CLDN18.2) expressionin normal tissues is restricted to cells of the gastric mucosa and is absent from other healthytissues However, CLDN 18.2 is expressed in 70% of primary gastric adenocarcinomas and itsmetastases (101). It has also been shown to be expressed in a number of other cancers,including pancreatic (50%), esophagus (30%) and NSCLC (25%)The restricted expressionmakes it a potential drug target for the treatment of gastric and pancreatic adenocarcinoma, asevidenced by efforts to target CLDN18.2 via naked antibody and CAR-T modalities.

* The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit of Lonza.

Synonyms

Claudin-18,isoform A2

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

产品概述

Applications	Binding assay or use as immunogen
Key Features	Clonality:Single clone Stability:Stable expression of CLDN18.2 for more than 15 passages

产品简介

Storage	Remove the frozen cells from the dry ice packaging and immediately placRemove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. We recommend immediate resuscitation upon receipt of the cells
QC	Mycoplasma negative
Stability	Stable expression of CLDN18.2 for more than 15 passages

培养条件

Freeze Medium	72% F12k, 20% FBS, 8% (V/V) DMSO
Culture Medium	F12K, 10% FBS, 500μg/ml Geneticin (G418 Sulfate)

图例

FACS analysis of CLDN18.2 expression in CHO-K1/ CLDN18.2 clone by antiCLDN18.2, compared with blank control (green line, untreated cell) and negative control (blue line, only treated with goat anti-human antibody). The cells analyzed are live cells (E1 gate).

Cell based ELISA of CLDN18.2 expression in CHO-K1/ CLDN18.2 clone. CHO-K1/CLDN18.2 cells are plated at 8E4 cells /well in 100μl DMEM plus with 10% FBS in 96-well plate overnight. Then fix with paraformaldehyde for 15 minutes. After washed, the cells are incubated with concentration gradient of IMAB-362 (anti-CLDN18.2 antibody) for 1 hour, and incubated with goat anti-human IgG Antibody (H&L) [HRP] antibody. After incubated with TMB, OD450 is detected by microplate reader

Immune assay of CLDN18.2 expression in CHO-K1/ CLDN18.2 clone. CHO-K1/ CLDN18.2 cells are plated at 5E5 cells /well in 100μl PBS in 96-well plate, incubated with concentration gradient of IMAB-362(anti-CLDN18.2 antibody) on ice for 1 hour. Then discard the supernatant and incubated with goat anti-human IgG antibody at 10 μg/ml on ice for 1 hour. Then analyzed on flow cytometer and analysis the mean fluorescence intensity of APC (Mean APC-H) in live cell gate (E1 in Figure 1).

Stability testing of CLDN18.2 expression in CHO-K1/ CLDN18.2 clone.4 for 22 passages by anti-CLDN18.2, compared with blank control (blue line, untreated cell) and negative control (purple line, treated with goat anti-human antibody)

CHO-K1/CLDN18.2 Stable Cell Line

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