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pAAV-Helper

The pHelper plasmid supplies essential adenoviral genes (E2A, E4, and VA RNA) to support AAV production in HEK293 cells, enabling efficient viral assembly without live adenovirus. It is used in combination with a Rep/Cap plasmid and a transgene plasmid (containing the gene of interest flanked by ITRs) to enable high-efficiency AAV packaging. For our catalog, we offer the pHelper plasmid at high-grade Tier 2 plasmid quality, optimized to support transfection studies. It is delivered in ≥80% supercoiled form with ≤0.01 EU/µg endotoxin, ensuring high purity and performance for AAV vector production.
RP-B00001
¥2933

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Description The pHelper plasmid supplies essential adenoviral genes (E2A, E4, and VA RNA) to support AAV production in HEK293 cells, enabling efficient viral assembly without live adenovirus.

Product Type AAV Helper Plasmid
Bacteria Resistance Ampicillin

Appearance Clear, colorless, and free from precipitates or foreign particles
Homogeneity (Supercoil) ≥80%
Endotoxin Level ≤0.01 EU/μg
Residual RNA Not detectable at 200 ng
E. coli Genomic DNA <15% of all bands via AGE
Restriction Analysis Conforming to reference pattern
Sequencing 100% Sequence Accuracy
Bio-burden Test No growth on agar plate after 72 hours

Storage Conditions Store at -20°C
Format 5mg/20mg in TE Buffer at 1mg/mL

Disclaimer For Research Use Only. Not for use in human therapeutics or diagnostic procedures.

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