| Description |
ssDNA_RAB11A_GFP_CTS HDR donor template is high purity GenExactTM ssDNA designed for inserting GFP into targeted RAB11A locus. Advantages of GenExactTM ssDNA donor template： ✔ Minimal cytotoxicity ✔ Precise knock-in, minimized off-target effect ✔ High purity and sequence verified ✔ Research to GMP grade Learn More>>> https://www.genscript.com/new-single-stranded-dna-synthesis-service.html This design also includes Cas9 Targeting Sequence (CTS) for improved gene knock-in efficiency. CTS allows for the co-delivery of donor DNA and sgRNA/Cas9 complex into the nucleus target gene site, therefore increasing HDR rate. More info for CTS design can be found in this paper collaborated with Dr. Marson at Gladstone and UCSF. This ssDNA HDR donor template needs to work in combination with SafeEdit Rab11a sgRNA, Cas9 protein, and RAB11A CTS Annealing Oligo. [Reference] Roth. et al. Reprogramming human T cell function and specificity with non-viral genome targeting. Nature 2018, 559(7714): 405-409. [Reference] Shy, et al. Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing. bioRxiv 2021.09.02.458799. |
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| Sequence |
AGCTGTCGTACTCGTCGTCGCGGGGTAGCTAGGAGTTCCAGGACTCAGTTTCCCCTTTGA GCCTCCTTTAGCGACTAAAGCTTGAAGCCCCACGCATCTCGACTCTCGCGCACACCGCCC TTGTTGGGCTCAGGGGCGGGGCGCCGCCCCCGGAAGTACTTCCCCTTAAAGGCTGGGGCC TGCCGGAAATGGCGCAGCGGCAGGGAGGGGCgCTTCACCCAGTCCGGCAGTTGAAGCTCG GCGCTCGGGTTACCCCTGCAGCGACGCCCCCTGGTCCCACAGATACCACTGCTGCTCCCG CCCTTTCGCTCCTCGGCCGCGCAATGGGCGGATCGGGTGGGACTAGTGGCAGCAAGGGCG AGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCC ACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGA AGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGA CCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCGCCACGACTTCTTCA AGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCAGCTTCAAGGACGACGGCA CCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGC TGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACT TCAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACT TCAAGATCCGCCACAACGTGGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGA ACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGT CCGTGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTgCTGGAgTTCGTGA CCGCCGCCGGGATCACTggaaccggtGCTggaagtggtACaCGCGACGACGAGTACGACT ACCTCTTTAAAGGTGAGGCCATGGGCTCTCGCACTCTACACAGTCCTCGTTCGGGGACCC GGGCCACTCCCGGTGGACCCTCGTGCCGGCCACCCCTGCACTGATATAGGCCTCCCTCAG CCCTTCCTTTTTGTGCGGTTCCGTCTCCTACCCAGCTCAGCCTCTTCTCCCCCGCTCAGA CAGGGGTCCCCATCACATGCCGCTCTCTGAGCGACCTCTCCATAGGCCTTCGCTGGCCTC AGAGCCCCTCCCTGCGTGTCCTTCCCCTGGCGGACTGCCTTCTCCCACATCGT |
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| Quantity | 50μg | ||||||||||||||
| Storage Condition | Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles. | ||||||||||||||
| Quality Specifications |
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