| Description |
ssDNA_TRAC_EGFP_CTS HDR donor template is high purity GenExactTM ssDNA designed for inserting EGFP into targeted TRAC locus. The resulting EGFP expression will be placed under the endogenous TCR promoter. Advantages of GenExactTM ssDNA donor template： ✔ Minimal cytotoxicity ✔ Precise knock-in, minimized off-target effect ✔ High purity and sequence verified ✔ Research to GMP grade Learn More>>> https://www.genscript.com/new-single-stranded-dna-synthesis-service.html This design also includes Cas9 Targeting Sequence (CTS) for improved gene knock-in efficiency. CTS allows for the co-delivery of donor DNA and sgRNA/Cas9 complex into the nucleus target gene site, therefore increasing HDR rate. More info for CTS design can be found in this paper collaborated with Dr. Marson at Gladstone and UCSF. This ssDNA HDR donor template needs to work in combination with SafeEdit TRAC sgRNA, Cas9 protein, and TRAC CTS Annealing Oligo. [Reference] Shy, et al. Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing. bioRxiv 2021.09.02.458799. |
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| Sequence |
gctttctctcagctggtacacggGGTTTCCTTGAGTGGCAGGCCAGGCCTGGCCGTGAAC GTTCACTGAAATCATGGCCTCTTGGCCAAGATTGATAGCTTGTGCCTGTCCCTGAGTCCC AGTCCATCACGAGCAGCTGGTTTCTAAGATGCTATTTCCCGTATAAAGCATGAGACCGTG ACTTGCCAGCCCCACAGAGCCCCGCCCTTGTCCATCACTGGCATCTGGACTCCAGCCTGG GTTGGGGCAAAGAGGGAAATGAGATCATGTCCTAACCCTGATCCTCTTGTCCCACAGATA TCCAGAACCCTGACCCTGCCGTGGGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGG CTGGAGACGTGGAGGAGAACCCTGGACCTatggtgagcaagggcgaggagctgttcaccg gggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgt ccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcacca ccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagt gcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccg aaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcg ccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgact tcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacg tctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccaca acatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcg acggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaag accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatca ctctcggcatggacgagctgtacaagtaaTGATCATAATCAAGCCATATCACATCTGTAG AGGTTTACTTGCTTTAAAAAACCTCCACACCTCCCCCTGAACCTGAAACATAAAATGAAT GCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGC ATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAA CTCATCAATGTATCTTATCATGTCTGGATCTGCACCAGCTGAGAGACTCTAAATCCAGTG ACAAGTCTGTCTGCCTATTCACCGATTTTGATTCTCAAACAAATGTGTCACAAAGTAAGG ATTCTGATGTGTATATCACAGACAAAACTGTGCTAGACATGAGGTCTATGGACTTCAAGA GCAACAGTGCTGTGGCCTGGAGCAACAAATCTGACTTTGCATGTGCAAACGCCTTCAACA ACAGCATTATTCCAGAAGACACCTTCTTCCCCAGCCCAGGTAAGGGCAGCTTTGGTGCCT TCGCAGGCTGTTTCCTTGCTTCAGGAATGGCCA |
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| Quantity | 50ug | ||||||||||||||
| Storage Condition | Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles. | ||||||||||||||
| Quality Specifications |
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