| Product Description |
The CytoSinct TCR αβ
Nanobeads, human is used for in vitro depletion of TCRα/β+ cells from
fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis
products or single cell suspension based on the surface expression of human
TCRα/β.
To begin the isolation, Nanobeads are added to the
cells. The cells/beads mixed suspension is loaded onto a CytoSinct Column
placed in the CytoSinct magnet, or other compatible columns with a magnet.
TCRα/β+ cells labeled with Nanobeads
are retained within the column and unlabelled cells (TCRα/β- cells)
are flushed out during the wash step. After removal of the magnetic field, the
retained TCRα/β+ cells can be eluted as the positively selected cell
fraction. |
| Capacity |
Cat. No.
| Product Name
| Size
| Capacity
|
|---|
L00897-1
| CytoSinct™ TCR αβ Nanobeads, human
| 1 mL
| Up to 1×109 total cells | | L00897-7.5 | CytoSinct™ TCR αβ Nanobeads, human
| 7.5 mL | Up to 7.5×109 total cells |
|
| Format |
Supplied
in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and
Poloxamer 188. |
| Components |
1 mL or 7.5 mL CytoSinct
TCR αβ Nanobeads, human
Nanobeads conjugated to monoclonal anti-human TCRα/β antibodies. |
| Storage |
Store at 2-8 °C. Do not
freeze. |
TCR αβ+ T cells were depleted from human PBMCs. TCRαβ+ cells were stained with anti-TCRα/β (clone: BW242/412) PE and gated on Live/hCD45+ cells.
For research use only. Not intended for human and animal therapeutic or diagnostic use.
