| Product Description |
The CytoSinct Streptavidin Nanobeads are
used for in vitro enrichment or depletion of cells labeled with one or a
cocktail of biotinylated antibody or ligand from fresh or frozen peripheral
blood mononuclear cells (PBMCs), leukapheresis products, or single-cell
suspension.
To begin the isolation, biotinylated
primary antibody or ligand are added to the cells for labeling, then CytoSinct
Streptavidin Nanobeads are added. The ceils/beads mixed suspension is loaded onto
a CytoSinct column placed in the CytoSinct magnet, or other compatible columns
with a magnet.
The cells labeled with Nanobeads are
retained within the column and unlabeled cells are flushed out during the wash
step. After removing the column from the magnetic field, the retained labeled cells
can be eluted as the positively selected cell fraction. |
| Format |
Supplied in water.
|
| Components |
1 mL or 0.5 mL CytoSinct Streptavidin Nanobeads
Nanobeads conjugated to streptavidin protein.
|
| Volume |
Cat. No.
| Name | Size | Capacity |
|---|
L00898-0.5
| CytoSinct™ Streptavidin Nanobeads
| 500 μL
| for up to 5×108
total cells
| | L00898-1 | CytoSinct™ Streptavidin Nanobeads | 1 mL | for up to 1×109
total cells |
|
| Storage |
Store at 2-8 °C. Do not freeze.
|
T cells were enriched from human PBMCs using CytoSinct Streptavidin Nanobeads (Cat. L00898) and biotinylated anti-human CD3 antibody. CD3+ cells were stained with anti-CD3 (clone: OKT3) BV785 and gated on Live/hCD45+.
For research use
only. Not intended for human and animal therapeutic or diagnostic use.
