T cells enriched by CytoSinct™ CD4 and CytoSinct™ CD8 nanobeads were activated by Enceed™ T cell Activation reagent. After 2 days, T cells were stained with CD25-PE and CD69-APC antibodies. Expression of CD25 and CD69 was analyzed by flow cytometry (Figure A).

T cells stained with carboxy fluorescein succinimidyl ester (CFSE) were stimulated by Enceed™ T cell Activation. After 7 days, proliferation was tested by flow cytometry.

Enceed™ T cell Activation, human

The Enceed T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation.

产品编号	L00899
规格	0.5 ml 1 ml 定制报价
数量
价格	￥2100


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产品概述

Product Description

The Enceed T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation.
The recommended titer of 1:200 has been found to achieve efficient stimulation of majority of T cell subsets. For special applications, the optimal stimulation titer should be determined experimentally. Excessive amount of the Enceed T cell Activation reagent may result in over-activation of T cells risking activation-induced cell death. Typically, the cells reach the exponential growth phase over 5 days and keep optimal expansion up to 13 days. Restimulation after 13 days can be performed for longer cultivation. The Enceed T cell Activation reagent can be used together with human IL-2, other cytokines such as human IL-15 and IL-7 can also be added for more efficient activation.

产品属性

Format

Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188.

Components

1 mL or 0.5 mL Enceed T cell Activation, human Nanobeads conjugated to monoclonal anti-human CD3 and anti-human CD28 antibodies

Volume

Cat. No.	Size	Capacity
L00899-0.5	500 μL	Up to 1×10⁸ T cells
L00899-1	1 mL	Up to 2×10⁸ T cells

Storage

Store at 2-8 °C. Do not freeze.

图例

T cells enriched by CytoSinct™ CD4 and CytoSinct™ CD8 nanobeads were activated by Enceed™ T cell Activation reagent. After 2 days, T cells were stained with CD25-PE and CD69-APC antibodies. Expression of CD25 and CD69 was analyzed by flow cytometry (Figure A).

T cells stained with carboxy fluorescein succinimidyl ester (CFSE) were stimulated by Enceed™ T cell Activation. After 7 days, proliferation was tested by flow cytometry.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Enceed™ T cell Activation, human

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