目录产品 » Enceed™ T cell Activation, human (GMP)

Enceed™ T cell Activation, human (GMP)

The Enceed T cell Activation, human (GMP) is used for in vitro activation and expansion of enriched T cells or peripheral blood mononuclear cells (PBMCs). Due to the nanoscale structure of Enceed T cell Activation, human (GMP), the excess reagent can be removed by centrifugation or conventional supernatant replacement.
L00935
询价

联系我们
产品概述
Product Description The Enceed T cell Activation, human (GMP) is used for in vitro activation and expansion of enriched T cells or peripheral blood mononuclear cells (PBMCs). Due to the nanoscale structure of Enceed T cell Activation, human (GMP), the excess reagent can be removed by centrifugation or conventional supernatant replacement.

产品属性
Capacity
Catalog number SizeCapacity
L009354 mLUp to 2×108 enriched T cells or up to 4×108 PBMCs in a maximal volume of 100 mL.
Components Colloidal solution of iron-dextran nanoparticles conjugated to monoclonal CD3 antibody and CD28 antibody in PBS buffer stabilized with HSA and Poloxamer 188
Endotoxin Content < 2 EU/mL as determined by Limulus Amebocyte Lysate (LAL) limit assay (USP <85>).
Sterility Sterility is tested and confirmed according to USP <71>.
Storage Store at 2-8 ℃.

Regulatory and legal notes
Disclaimer Enceed T cell Activation, human (GMP) is for ex vivo cell processing only, and are not intended for human in vivo applications.
It is designed following the recommendations of USP <1043> on ancillary materials and the Guidance for CMC Research and Evaluation of Immune Cell Therapy Products (Trial Implementation) published by the National Medical Products Administration in China. It is manufactured and tested according to relevant GMP guidelines.
Warranty The products sold hereunder are warranted only to be free from defects in workmanship and material at the time of delivery to the customer. GenScript makes no warranty or representation, either expressed or implied, with respect to the fitness of a product for a particular purpose. There are no warranties, expressed or implied, which extend beyond the Specification of the products.
GenScript's liability is limited to either replacement of the products or refund of the purchase price.
GenScript is not liable for any property damage, personal injury, or economic loss caused by the products.

Warnings and precautions
Warnings and precautions 1. Do not freeze.
2. The product is intended for in vitro use only. Not for intravenous infusion.
3. When using this product, the national legislation and regulations must be followed. Any application of ex vivo processed target cells is exclusively within the responsibility of the user.
4. Do not use after the expiry date printed on the product label.
5. Do not use it if the package is damaged. Use reagent only if the vial is undamaged and sealed.
6. Do not reuse.
7. The product is not recommended for use with patients known or suspected to have sensitivity against mouse immunoglobulins or iron-dextran.
8. Patients may develop human anti-mouse antibodies (HAMA).

Instructions for use
Instructions for use 1. The cell concentration and titer for stimulation of fresh PBMCs or enriched T cells are recommended in the following table. For optimal stimulation T cells should be activated with 1×106 T cells per mL when used at recommended titer 1:25. For FPBMC optimal stimulation conditions are 2×106 PBMCs per mL with a recommended titer 1:25.
Enriched T cells Cell concentration
Titer
Optimum
1×106 per mL1:25
Maximum
2×106 per mL1:25

PBMCs
Cell concentrationTiter
Optimum
2×106 per mL1:25
Maximum
4×106 per mL1:25
2. When working with frozen material, it is recommended to rapidly thaw the cells, wash away the freezing solution containing DMSO, resuspend the cells in culture medium at densities below 5×106 cells / mL, and rest the cells overnight at 37°C and 5% CO2 in culture medium.
3. Presence of residual EDTA will hamper T cell stimulation1. Ensure removal of EDTA prior to T cell stimulation with Enceed T cell Activation, human (GMP) e.g.via a washing step.
4. Enceed T cell Activation, human (GMP) can be used in combination with Retro- or Lentiviral transduction. It is recommended to transduction T cells 2-3 days after stimulation.
5.Work under sterile conditions.
6. Remove the reagent from the vial manually or use an automated process.

喜欢新升级的网站吗？

讨厌

不喜欢

一般

喜欢

非常喜欢

*
Skip