| Product Description |
The
CytoSinct CD19 Nanobeads, human, are used for in vitro enrichment or depletion of
CD19+ cells from fresh or frozen
peripheral blood mononuclear cells (PBMCs), leukapheresis products, or
single-cell suspension based on the surface expression of human CD19.
To begin the isolation, Nanobeads are added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct Column placed in the CytoSinct Magnet, or other compatible columns with a magnet.
The CD19+ cells labeled with Nanobeads are retained within the
column and unlabeled cells (CD19- cells) are flushed out during the wash step. After
removing the column from the magnetic field, the retained CD19+ cells can be eluted as the
positively selected cell fraction.
|
| Capacity |
| Cat. No. | Product Name | Size | Capacity |
|---|
| L00957-0.5 | CytoSinct™ CD19 Nanobeads, human | 500 μL | Up to 5 × 108 total cells | | L00957-1 | CytoSinct™ CD19 Nanobeads, human | 1 mL | Up to 1 × 109 total cells |
|
| Format |
Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188. |
| Components |
1 mL or 0.5 mL CytoSinct CD19 Nanobeads, human
Nanobeads conjugated to monoclonal anti-human CD19 antibodies (isotype: chimeric IgG1). |
| Storage |
Store at 2 - 8 °C. Do not freeze. |
CD19+ B cells were enriched or depleted from human PBMCs. CD19+ cells were stained with antiCD19 (clone: 4G7) PE and gated on Live/hCD45+.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
