| Product Description |
The CytoSinct CD34 Nanobeads, human are
used for in vitro enrichment CD34+ cells from fresh or
frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or
single cell suspension based on the surface expression of human CD34.
To begin the isolation, Nanobeads are
added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct
Column placed in the CytoSinct magnet, or other compatible columns with a
magnet.
The CD34+ cells labeled with
Nanobeads are retained within the column and unlabeled cells (CD34 cells) are
flushed out during the wash step. After removing the column from the magnetic
field, the retained CD34+ cells can be eluted as the positively
selected cell fraction. |
| Format |
Supplied in phosphate buffered-saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188.
|
| Components |
1 mL CytoSinct CD34 Nanobeads, human
Nanobeads conjugated to monoclonal anti-human CD34 antibodies (isotype: chimeric IgG1).
|
| Volume |
| Cat. No. | Name | Size | Capacity |
|---|
| L00967-1 | CytoSinct™ CD34 Nanobeads, human | 1 mL | for up to 1×109 total cells |
|
| Storage |
Store at 2-8 °C. Do not freeze.
|
CD34+ cells were enriched from human single cell suspension. CD34+ cells were stained with anti-CD34 (clone: 581) PE and gated on Live/hCD45+ cells.
For research use only. Not intended for human and animal
therapeutic or diagnostic use.
