CHO-K1/CD32A 131Arg Stable Cell Line

Product Description

Recombinant CHO-K1 cells stably overexpress immunoglobulin gamma Fc region receptor II-a (FcγRIIa / CD32A 131Arg). The surface expression of CD32A 131Arg is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of antibody based biologics binding with CD32A 131Arg. GenScript offers both CD32A 131Arg stable cell line and CD32A 131His stable cell line (Cat. No. M00589) for FcγRIIa polymorphism study.

Applications

Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon optimized from NM_001136219.1; no expressed tags
Target Protein	NP_001129691.1
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1X10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	F-12K (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	F-12K, 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

技术背景

Gene Synonyms

CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1

Background

Human CD32 group consists of FcγRIIA, B and C proteins that share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32A, also known as FcγRIIA, is a surface receptor protein and part of a large population of B cell co-receptors. The protein is found on phagocytic cells such as macrophages and neutrophils, and it is involved in the process of phagocytosis and clearing of immune complexes. It has low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loop acts to lower the production of IgG by B cells when there is a surplus in the body. Therefore, targeting CD32A may represent an approach to immunotherapy of B-cell malignancies.

图例

Figure 1. FACS analysis of cell surface expression of CD32A 131Arg on CHO-K1 cells.
CHO-K1/CD32A 131Arg cells (red) and negative control CHO-K1 cells (blue) were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 303208, Biolegend).

Figure 2: FACS analysis of cell surface expression of CD32A 131Arg on CHO-K1 cells from different passages.
CHO-K1/CD32A 131Arg cells and negative control CHO-K1 cells were probed using APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Biosciences). P10, P17, P22, and P27 represent cell passage numbers.

产品编号	M00887
规格	2 vials 大单询价
数量
价格	询价


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