目录产品 » HT1080/SBE-Luc2P
TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line

Figure 1: Functional evaluation of HT1080/SBE-Luc cells by TGF-β1, TGF-β2 and TGF-β3.
HT1080/SBE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of different concentrations of TGF-β1 (GenScript, Z03411), TGF-β2 (GenScript, Z03429) and TGF-β3. (GenScript, Z03430). After 6 hours of incubation, Fire-Lumi™ Reagent (GenScript, L00877C) was added and the luminescence signal was measured by Thermo Scientific Varioskan LUX. The relative light unit (RLU) were recorded and normalized to plot against the log of the cumulative doses of TGF-β (mean ± SD, n = 2). The EC50 of TGF-β on HT1080/SBE-Luc cells were 0.096 ng/ml (TGF-β1), 0.058 ng/ml (TGF-β2), 0.075 ng/ml (TGF-β3).

TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line

Figure 2: Functional evaluation of HT1080/SBE-Luc cells by M7824.
HT1080/SBE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours. Mix TGF-β1 (0.3 ng/ml), TGF-β2 (0.3 ng/ml) or TGF-β3 (0.3 ng/ml) with different concentrations of M7824 for 30 min. After 30 min of incubation, the mixture was added into the 384-well plate and incubated at 37℃ for 6 hours. After incubation, Fire-Lumi™ Reagent (GenScript, L00877C) was added and luminescence measured by Thermo Scientific Varioskan LUX. The relative light unit (RLU) were recorded and normalized to plot against the log of the cumulative doses of TGF-β (mean ± SD, n = 2). The IC50 of M7824 on HT1080/SBE-Luc cells were 0.02 μg/ml (TGF-β1), 2.58 μg/ml (TGF-β2), 0.002 μg/ml (TGF-β3).

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X) *Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line

Recombinant HT1080 SBE reporter stable cell line expresses luciferase in response to activation of TGFβ/SMAD signaling pathway. This stable cell line product is designed for monitor TGF signaling pathway activity and screen for activators or inhibitors of the TGF/SMAD signaling pathway.
M00902
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产品概述
Product Description Recombinant HT1080 SBE reporter stable cell line expresses luciferase in response to activation of TGFβ/SMAD signaling pathway. This stable cell line product is designed for monitor TGF signaling pathway activity and screen for activators or inhibitors of the TGF/SMAD signaling pathway.
Applications Monitor TGF signaling pathway activity and screen for activators or inhibitors of the TGF/SMAD signaling pathway

产品简介
Host Cell HT1080
Quantity Two vials of frozen cells (>1×106 per vial in 1 ml)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium MEM (Cat. No. 32561-037, Gibco), 10% FBS (Cat. No. 10099-141C, Gibco)
Culture Medium MEM, 10% FBS, 500 μg/ml geneticin (Cat. No. 10131-035, Gibco)

技术背景
Gene Synonyms SBE reporter, TGFβ reporter, TGF beta reporter, SMAD reporter
Background The transforming growth factor beta (TGF-β) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGF-β signaling has been linked to cardiac disease, cancer, Alzheimer’s and other human diseases. TGF-β proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocate to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGF-β/ SMAD responsive genes. 

图例
  • TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line
    • TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line

    Figure 1: Functional evaluation of HT1080/SBE-Luc cells by TGF-β1, TGF-β2 and TGF-β3.
    HT1080/SBE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of different concentrations of TGF-β1 (GenScript, Z03411), TGF-β2 (GenScript, Z03429) and TGF-β3. (GenScript, Z03430). After 6 hours of incubation, Fire-Lumi™ Reagent (GenScript, L00877C) was added and the luminescence signal was measured by Thermo Scientific Varioskan LUX. The relative light unit (RLU) were recorded and normalized to plot against the log of the cumulative doses of TGF-β (mean ± SD, n = 2). The EC50 of TGF-β on HT1080/SBE-Luc cells were 0.096 ng/ml (TGF-β1), 0.058 ng/ml (TGF-β2), 0.075 ng/ml (TGF-β3).

  • TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line
    • TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line

    Figure 2: Functional evaluation of HT1080/SBE-Luc cells by M7824.
    HT1080/SBE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours. Mix TGF-β1 (0.3 ng/ml), TGF-β2 (0.3 ng/ml) or TGF-β3 (0.3 ng/ml) with different concentrations of M7824 for 30 min. After 30 min of incubation, the mixture was added into the 384-well plate and incubated at 37℃ for 6 hours. After incubation, Fire-Lumi™ Reagent (GenScript, L00877C) was added and luminescence measured by Thermo Scientific Varioskan LUX. The relative light unit (RLU) were recorded and normalized to plot against the log of the cumulative doses of TGF-β (mean ± SD, n = 2). The IC50 of M7824 on HT1080/SBE-Luc cells were 0.02 μg/ml (TGF-β1), 2.58 μg/ml (TGF-β2), 0.002 μg/ml (TGF-β3).

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X) *Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.


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