目录产品 » HT1080/SBE-Luc2P
Manual
COAs

TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line

Recombinant HT1080 cells stably contain the luciferase reporter gene. HT1080 cell line is derived from human fibrosarcoma biopsy. The luciferase reporter gene was inserted into the genome of this cell and can be translated under the control of Smad - binding element (SBE). The cytokine samples (TGF - β) can activate the receptor and transmit this signal to the downstream signaling pathways. This stable cell line product is designed for In Vitro cell - based luciferase assays to determine the bioactivity of TGF - β or corresponding antibodies.
询价
M00902

产品简介
Product Description Recombinant HT1080 cells stably contain the luciferase reporter gene. HT1080 cell line is derived from human fibrosarcoma biopsy. The luciferase reporter gene was inserted into the genome of this cell and can be translated under the control of Smad - binding element (SBE). The cytokine samples (TGF - β) can activate the receptor and transmit this signal to the downstream signaling pathways. This stable cell line product is designed for In Vitro cell - based luciferase assays to determine the bioactivity of TGF - β or corresponding antibodies.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
返回

培养条件
Culture Medium MEM, 10% FBS, 500 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium MEM (Cat. No. 41090, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
返回

图例
TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line

Figure 1. TGF-β-induced luciferase expression in HT1080/SBE-Luc cells. After stimulation by agonist TGF-β for 6 hours, the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Varioskan Lux, Thermo Fisher) and plotted against the log of the cumulative doses of TGF-β (Mean ± SEM, n = 2). (TGF-β1, Cat. No. Z03411, Genscript; TGF-β2, Cat. No. Z03429, Genscript; TGF-β3, Cat. No. Z03430, Genscript)

»

TGF-β/SMAD Signaling Pathway SBE Reporter-HT1080 Cell Line

Figure 2. Dose response of M7824 (TGF-β trap) to block the TGF-β-induced luminescence in HT1080/SBE-Luc cell. After stimulation with agonist TGF-β for 6 hours, inhibition effect of M7824 on this cell are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript). The relative luminescence units (RLU) were recorded by plate reader (Varioskan Lux, Thermo Fisher) and plotted against the log of the cumulative doses of M7824 (Mean ± SEM, n = 2). »

<
>

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.