目录产品 » HEK293/SBE-Luc2P
TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line

Figure 1. TGF-β-induced luciferase expression in HEK293/SBE-Luc cells. After stimulation by TGF-β (Z03411, Z03429, Z03430, Genscript), the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Varioskan LUX, Thermo Fisher). The RLU were plotted against the log of the cumulative doses of TGF-β (Mean ± SEM, n = 3).

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RLU and starts at Bottom and goes to Top along a sigmoid curve.

TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line

Figure 2. Functional evaluation of M7824, the TGFR fusion protein, in HEK293/SBE-Luc cells. HEK293/SBE-Luc cells were dispensed into the microplate and incubated at 37℃ overnight prior to the addition of different concentrations of M7824. After 30 minutes’ incubation, TGF-β was added into the plate and incubated at 37℃ for 6 hours. Luminescence signal was detected with the plate reader (Varioskan LUX, Thermo Fisher).

TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line

HEK293/SBE-Luc2P is designed for monitoring the TGF-β/SMAD signaling pathway activity and used to screen activators or inhibitors of TGF-β/SMAD signaling pathway.
M00903
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产品简介
Product Description Recombinant HEK293 cells stably overexpress the luciferase reporter gene under the control of Smad binding element (SBE). This stable cell line product is designed for measuring the In Vitro biological potency of TGF or antibodies.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium DMEM, 10% FBS, 250 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium DMEM (Cat. No. 10569-010, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% compete growth medium, 5% DMSO (Cat. No. D2650, Sigma).

图例
  • TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line
    • TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line

    Figure 1. TGF-β-induced luciferase expression in HEK293/SBE-Luc cells. After stimulation by TGF-β (Z03411, Z03429, Z03430, Genscript), the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Varioskan LUX, Thermo Fisher). The RLU were plotted against the log of the cumulative doses of TGF-β (Mean ± SEM, n = 3).

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is RLU and starts at Bottom and goes to Top along a sigmoid curve.

  • TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line
    • TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line

    Figure 2. Functional evaluation of M7824, the TGFR fusion protein, in HEK293/SBE-Luc cells. HEK293/SBE-Luc cells were dispensed into the microplate and incubated at 37℃ overnight prior to the addition of different concentrations of M7824. After 30 minutes’ incubation, TGF-β was added into the plate and incubated at 37℃ for 6 hours. Luminescence signal was detected with the plate reader (Varioskan LUX, Thermo Fisher).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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