TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line

Figure 1: TGF-β1, TGF-β2 or TGF-β3 induced concentration-dependent stimulation of intracellular luciferase accumulation in HEK293/SBE-Luc cells.
HEK293/SBE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of different concentrations of TGF-β1 (Cat. No. Z03411, GenScript), TGF-β2 (Cat. No. Z03429, GenScript) and TGF-β3. (Cat. No. Z03430, GenScript). After 6 hours of incubation, Fire-Lumi™ Reagent (Cat. No. L00877C, GenScript) was added and the luminescence signal was measured by Thermo Scientific Varioskan LUX. The relative light unit (RLU) were recorded and normalized to plot against the log of the cumulative doses of TGF-β (mean ± SD, n = 2). The EC₅₀ of TGF-β1, TGF-β2, TGF-β3 on HEK293/SBE-Luc cells were 0.095 ng/ml, 0.096 ng/ml, 0.135 ng/ml, respectively.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

Figure 2: M7824 induced blockade of intracellular luciferase accumulation triggered by TGF-β1, TGF-β2 or TGF-β3 in HEK293/SBE-Luc cells.
HEK293/SBE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours. Mix TGF-β1 (0.3 ng/ml), TGF-β2 (0.3 ng/ml) or TGF-β3 (0.3 ng/ml) with different concentrations of M7824 for 30 min. After 30 min of incubation, the mixture was added into the 384-well plate and incubated at 37℃ for 6 hours. After incubation, Fire-Lumi™ Reagent (Cat. No. L00877C, GenScript) was added and luminescence measured by Thermo Scientific Varioskan LUX. The relative light unit (RLU) were recorded and normalized to plot against the log of the cumulative doses of TGF-β (mean ± SD, n = 2). The IC₅₀ of M7824 on HEK293/SBE-Luc cells were 0.0048 μg/ml (TGF-β1 activation), 1.221 μg/ml (TGF-β2 activation), 0.0005 μg/ml (TGF-β3 activation).

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line

HEK293/SBE-Luc2P is designed for monitoring the TGF-β/SMAD signaling pathway activity and used to screen activators or inhibitors of TGF-β/SMAD signaling pathway.

产品编号	M00903
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant HEK293 SBE reporter stable cell line expresses luciferase in response to activation of TGFβ/SMAD signaling pathway. This stable cell line product is designed for monitor TGF signaling pathway activity and screen for activators or inhibitors of the TGF/SMAD signaling pathway.
Culture Properties	Adherent
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	DMEM, 10% FBS, 250 μg/ml geneticin (Cat. No.10131-035, Gibco)
Complete Growth Medium	DMEM (Cat. No. 10569-010, Gibco), 10% FBS (Cat. No. 10099-141C, Gibco)
Freeze Medium-DATA	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1: TGF-β1, TGF-β2 or TGF-β3 induced concentration-dependent stimulation of intracellular luciferase accumulation in HEK293/SBE-Luc cells.
HEK293/SBE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of different concentrations of TGF-β1 (Cat. No. Z03411, GenScript), TGF-β2 (Cat. No. Z03429, GenScript) and TGF-β3. (Cat. No. Z03430, GenScript). After 6 hours of incubation, Fire-Lumi™ Reagent (Cat. No. L00877C, GenScript) was added and the luminescence signal was measured by Thermo Scientific Varioskan LUX. The relative light unit (RLU) were recorded and normalized to plot against the log of the cumulative doses of TGF-β (mean ± SD, n = 2). The EC₅₀ of TGF-β1, TGF-β2, TGF-β3 on HEK293/SBE-Luc cells were 0.095 ng/ml, 0.096 ng/ml, 0.135 ng/ml, respectively.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

Figure 2: M7824 induced blockade of intracellular luciferase accumulation triggered by TGF-β1, TGF-β2 or TGF-β3 in HEK293/SBE-Luc cells.
HEK293/SBE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours. Mix TGF-β1 (0.3 ng/ml), TGF-β2 (0.3 ng/ml) or TGF-β3 (0.3 ng/ml) with different concentrations of M7824 for 30 min. After 30 min of incubation, the mixture was added into the 384-well plate and incubated at 37℃ for 6 hours. After incubation, Fire-Lumi™ Reagent (Cat. No. L00877C, GenScript) was added and luminescence measured by Thermo Scientific Varioskan LUX. The relative light unit (RLU) were recorded and normalized to plot against the log of the cumulative doses of TGF-β (mean ± SD, n = 2). The IC₅₀ of M7824 on HEK293/SBE-Luc cells were 0.0048 μg/ml (TGF-β1 activation), 1.221 μg/ml (TGF-β2 activation), 0.0005 μg/ml (TGF-β3 activation).

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

TGF-β/SMAD Signaling Pathway SBE Reporter-HEK293 Cell Line

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