Figure 1: Functional evaluation of HEK293/SRE-Luc cells by EGF. HEK293/SRE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of different concentrations of EGF (GenScript, Z02691). After 6 hours of incubation, Fire-Lumi^TM Reagent (GenScript, L00877) was added and luminescence measured by Thermo Scientific Varioskan LUX.

Figure 2: Functional evaluation of HEK293/SRE-Luc cells by Cetuximab. HEK293/SRE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of EGF (0.03 µg/ml, GenScript). After 30 min of incubation, different concentrations of Cetuximab were added into the 384-well plate and incubated at 37℃ for 6 hours. After 6 hours of incubation, Fire-Lumi^TM Reagent (GenScript, M00877) was added and luminescence measured by Thermo Scientific Varioskan LUX.

Figure 3: Functional evaluation of HEK293/SRE-Luc cells by FGF. HEK293/SRE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of different concentrations of FGF (GenScript, Z03116-50). After 6 hours of incubation, Fire-Lumi^TM Reagent (GenScript, L00877) was added and luminescence measured by Thermo Scientific Varioskan LUX.

HEK293/SRE-Luc Stable Cell Line

The HEK293/SRE-Luc cell line is designed for measuring the potency of EGF or anti-EGFR, and monitoring MAPK/ERK signaling pathway activity.

产品编号	M00927
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Human Recombinant HEK293/SRE-Luc Stable Cell Line stably overexpress the luciferase reporter (Luc) gene. This stable cell line product is designed for measuring the function of EGF or anti-EGFR.
Culture Properties	Adherent
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	DMEM, 10% FBS, 300 μg/ml Geneticin (Cat. # 10131-035, Gibco)
Complete Growth Medium	DMEM (Cat. # 10569-010, Gibco), 10% FBS (Cat. # 10091-148, Gibco)
Freeze Medium-DATA	90%FBS (Cat. No. 10091-148, Gibco), 10% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1: Functional evaluation of HEK293/SRE-Luc cells by EGF. HEK293/SRE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of different concentrations of EGF (GenScript, Z02691). After 6 hours of incubation, Fire-Lumi^TM Reagent (GenScript, L00877) was added and luminescence measured by Thermo Scientific Varioskan LUX.

Figure 2: Functional evaluation of HEK293/SRE-Luc cells by Cetuximab. HEK293/SRE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of EGF (0.03 µg/ml, GenScript). After 30 min of incubation, different concentrations of Cetuximab were added into the 384-well plate and incubated at 37℃ for 6 hours. After 6 hours of incubation, Fire-Lumi^TM Reagent (GenScript, M00877) was added and luminescence measured by Thermo Scientific Varioskan LUX.

Figure 3: Functional evaluation of HEK293/SRE-Luc cells by FGF. HEK293/SRE-Luc cells were plated and incubated at 37 ℃ for 16-18 hours prior to the addition of different concentrations of FGF (GenScript, Z03116-50). After 6 hours of incubation, Fire-Lumi^TM Reagent (GenScript, L00877) was added and luminescence measured by Thermo Scientific Varioskan LUX.

For research use only. Not intended for human and animal therapeutic or diagnostic use.

HEK293/SRE-Luc Stable Cell Line

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