Figure 1. Luminescence analysis of activation of STAT6-RE-Luc and IL-4R on HEK293 cells. The HEK293/STAT6-RE-Luc cells were stimulated by IL-4 (Cat. No. Z02925-50, Genscript). Fire-Lumi™ Reagent (Cat. No. L00877C-1000, Genscript) was added and luminescence was measured by PHERAstar FSX.

Figure 2. Luminescence analysis of activation of STAT6-RE-Luc and IL-13R on HEK293 cells. The HEK293/STAT6-RE-Luc cells were stimulated by IL-13 (Cat. No. Z03020-50, Genscript). Fire-Lumi™ Reagent (Cat. No. L00877C-1000, Genscript) was used and luminescence measured by PHERAstar FSX.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RLU and starts at Bottom and goes to Top along a sigmoid curve.

HEK293/STAT6-RE-Luc Stable Cell Line

This is a cell model with luciferase repoter gene for cell-based assay. HEK293/STAT6-RE-Luc Stable cell line is designed for In Vitro assay to detect the bioactivity of IL-4 IL-13. The binding affininity or the blockade potency of the antibody drug can also be evaluated with this cell model.

产品编号	M00949
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant HEK293 cells stably express STAT6-RE-Luc and human interleukin 4 Receptor (IL-4R) on the surface and activate the receptor to the downstream signaling pathways upon the binding of IL-4 and IL-13. The surface expression of IL-4R is validated by luminescence analysis. This stable cell line product is designed for cell-based functional assays that measure binding affinity and stability of antibody based biologics binding with IL-4 and IL-13.
Culture Properties	Adherent
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Size	2 vials of frozen cells (>1X10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	DMEM, 10% FBS, 8 μg/ml Puromycin (Cat. No.A11138-02, Gibco), 300 μg/ml Geneticin (Cat. No.10131-027, Gibco), 200 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen), 100 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium	DMEM, 10% FBS
Freeze Medium-DATA	42% DMEM (Cat. No.10569-010, Gibco), 50% FBS (Cat. No.10099-141, Gibco), 8% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1. Luminescence analysis of activation of STAT6-RE-Luc and IL-4R on HEK293 cells. The HEK293/STAT6-RE-Luc cells were stimulated by IL-4 (Cat. No. Z02925-50, Genscript). Fire-Lumi™ Reagent (Cat. No. L00877C-1000, Genscript) was added and luminescence was measured by PHERAstar FSX.

Figure 2. Luminescence analysis of activation of STAT6-RE-Luc and IL-13R on HEK293 cells. The HEK293/STAT6-RE-Luc cells were stimulated by IL-13 (Cat. No. Z03020-50, Genscript). Fire-Lumi™ Reagent (Cat. No. L00877C-1000, Genscript) was used and luminescence measured by PHERAstar FSX.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RLU and starts at Bottom and goes to Top along a sigmoid curve.

For research use only. Not intended for human and animal therapeutic or diagnostic use.

HEK293/STAT6-RE-Luc Stable Cell Line

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