CHO-K1/Spike/LTR-Luc cell pool cells were plated and incubated at 37°C, 5% CO₂ for 16-20 hours before the addition of increasing concentrations of anti-Spike neutralizing antibody, followed by 1-hour incubation. Subsequently, HEK293/ACE2/Tat cell pool cells were added. After a 4-hour incubation, luciferase substrate reagent was introduced, and luminescence was measured using the PHERAstar system. The data were analyzed using GraphPad Prism® software.

HEK293/ACE2/Tat

HEK293 cells overexpressing ACE2 and Tat protein for application of cell fusion assay

产品编号	RD00885
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	HEK293 cells overexpressing ACE2 and Tat protein for application of cell fusion assay
Paired Cat. No	RD00902
Culture Properties	Adherent
Product Type	Cell pool
Size	2 vials of frozen cells (>1X10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	DMEM, 10% FBS, 1 μg/mL Puromycin, 5 μg/mL Blasticidin
Complete Growth Medium	DMEM, 20% FBS
Freeze Medium-DATA	90% Complete Growth Medium, 10% DMSO

图例

CHO-K1/Spike/LTR-Luc cell pool cells were plated and incubated at 37°C, 5% CO₂ for 16-20 hours before the addition of increasing concentrations of anti-Spike neutralizing antibody, followed by 1-hour incubation. Subsequently, HEK293/ACE2/Tat cell pool cells were added. After a 4-hour incubation, luciferase substrate reagent was introduced, and luminescence was measured using the PHERAstar system. The data were analyzed using GraphPad Prism® software.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

HEK293/ACE2/Tat

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