Flow cytometric analysis of CHO-K1/Spike/LTR-Luc stained with ACE2 Protein (Sino Biological, Cat. No.: 10108-H05H) after cell digestion using Accutase Cell Dissociation Reagent.
CHO-K1/Spike/LTR-Luc cell pool cells were plated and incubated at 37°C, 5% CO2 for 16-20 hours before the addition of increasing concentrations of anti-Spike neutralizing antibody, followed by 1-hour incubation. Subsequently, HEK293/ACE2/Tat cell pool cells were added. After a 4-hour incubation, luciferase substrate reagent was introduced, and luminescence was measured using the PHERAstar system. The data were analyzed using GraphPad Prism® software.
CHO-K1/Spike/LTR-Luc
CHO-K1 cells overexpressing Spike protein and luciferase driven by HIV-1 LTR for application of binding screening or cell fusion assay
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