CPF1/Cas12a mRNA (Cap1, m1Ψ)

Cpf1 is a novel class of CRISPR-Cas DNA endonuclease. The LbCpf1/Cas12a allow efficient mutagenesis in zebrafish and Xenopus (Moreno-Mateos, et al. Nat Communication, 2017; 8: 2024). LbCpf1 increases homology-directed genome editing. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudo Uridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.