eGFP Self-Amplifying RNA (Cap1, m5C)

The EGFP self-amplifying RNA (saRNA) expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP saRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects. The expressed protein has an excitation wavelength of 488nm, emission at 507nm. This saRNA is capped with Cap1-AU structure with high capping efficiency. It has 100% substituted with 5-methylcytidine for enhanced expression and reduced immunogenicity. The saRNA has a 68A tail in its sequence, mimics a mature mRNA.