GenLNP-S01-eSpCas9 mRNA(m1Ψ)-TRAC sgRNA

The LNP formulation loaded with eSpCas9 mRNA (m1Ψ) and TRAC sgRNA is prepared using generic LNP formulation with main ionizable lipid of SM102. The payload eSpCas9 mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudo-Uridine. HPLC purified SafeEdit sgRNA with modifications targeting the first exon of the constant chain of the TCRα gene (TRAC), which enhances CAR-T cell potency and persistence by utilizing the endogenous transcriptional control of TCR gene. The eSpCas9 mRNA and TRAC sgRNA are loaded at a molar ratio of 1:10 in this LNP formulation. This formulation is a good control for testing if SM102 LNP formulation is a good potential formulation for your CRISPR gene know out product under research in your in vitro and in vivo experiment model.